(Invitrogen) . . B) M19 CHO cells were cultivated in the absence or presence of sterols supplied from DMSO as indicated and the protein amounts determined. Total and membrane cholesterol content (BODIPY-cholesterol and CTxB) analysis. . Cholesterol, an essential component of membranes, regulates membrane fluidity and thus affects various receptor-mediated signal transduction pathways. The absorption and emission bands of DiI-C 12 (in DMSO) are centered at 551 nm and 659 nm, respectively (Fig. Steroids and derivatives 10124102 Print Invitrogen™ CholEsteryl 4,4-Difluoro-5- (4-Methoxyphenyl)-4-Bora-3a,4a-Diaza- s -Indacene-3-Undecanoate (CholEsteryl BODIPY™ 542/563 C 11 ) Orange-red fluorescent cholesteryl ester can be used as tracer of cholesterol transport and rand as general nonexchangeable membrane marker Brand: Invitrogen™ C12680 bodipy-cholesterol is a biologically active and cell-permeable analog of cholesterol that is tagged with a fluorescent bodipy group at carbon 24. Glycolipid Trafficking in Drosophila Undergoes Pathway Switching in ... day with BODIPY-labeled LDL (Invitrogen). Labelingmedium wasprepared throughmixing unlabeled cholesterol (0.025mM),BODIPY- cholesterol (0.1mM) andcyclo- bodipy-cholesterol is a biologically active and cell-permeable analog of cholesterol that is tagged with a fluorescent bodipy group at carbon 24. New BODIPY lipid probes for fluorescence studies of membranes BODIPY or boron dipyrromethene is a class of strong ultraviolet-absorbing molecules demonstrating a relatively sharp emission peak. This . Defective AMPK regulation of cholesterol metabolism accelerates ... An apoA-I mimetic peptide facilitates off-loading cholesterol from HDL ... New BODIPY lipid probes for fluorescence studies of membranes PDF Synergism between albumin, bicarbonate and cAMP upregulation for ...